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KMID : 0032220150270040364
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Annals of Dermatology
2015 Volume.27 No. 4 p.364 ~ p.370
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Protease-Activated Receptor-2 Is Associated with Terminal Differentiation of Epidermis and Eccrine Sweat Glands
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Shin Yong-Sup
Kim Hyung-Won
Kim Chang-Deok
Kim Hyun-Woo
Park Jin-Woon
Jung Sung-Gyun
Lee Jeung-Hoon
Ko Young-Kwon
Lee Young-Ho
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Abstract
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Background: Protease-activated receptor 2 (PAR-2) participates in various biological activities, including the regulation of epidermal barrier homeostasis, inflammation, pain perception, and melanosome transfer in the skin.
Objective: To evaluate the basic physiological role of PAR-2 in skin.
Methods: We investigated PAR-2 expression in human epidermis, skin tumors, and cultured epidermal cells using western blot and immunohistochemical analysis. Additionally, we examined the effect of the PAR-2 agonist, SLIGRL-NH2, on cultured keratinocytes.
Results: Strong PAR-2 immunoreactivity was observed in the granular layer of normal human skin and the acrosyringium of the eccrine sweat glands. In contrast, weak PAR-2 immunoreactivity was seen in the granular layer of callused skin and in the duct and gland cells of the eccrine sweat glands. Interestingly, PAR-2 immunoreactivity was very weak or absent in the tumor cells of squamous cell carcinoma (SCC) and syringoma. PAR-2 was detected in primary keratinocytes and SV-40T-transformed human epidermal keratinocytes (SV-HEKs), an immortalized keratinocyte cell line, but not in SCC12 cells. SV-HEKs that were fully differentiated following calcium treatment displayed higher PAR-2 expression than undifferentiated SV-HEKs. Treatment of cultured SV-HEKs with PAR-2 agonist increased loricrin and filaggrin expression, a terminal differentiation marker.
Conclusion: Our data suggest that PAR-2 is associated with terminal differentiation of epidermis and eccrine sweat glands. (Ann Dermatol 27(4) 364∼370, 2015)
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KEYWORD
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Eccrine sweat glands, Epidermis, Keratinocytes, Terminal differentiation
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